Extended Data Fig. 2: Effect of ARID1A depletion on YAP/TAZ levels, localization and activity.
From: The SWI/SNF complex is a mechanoregulated inhibitor of YAP and TAZ
a, Results of luciferase assays with the 8×GTIIC-Lux reporter in HEK293 cells transfected with empty or YAP-expressing vectors and the indicated siRNAs. Data are normalized to control siRNA- and empty vector-transfected cells and are presented as mean + s.d. of n = 3 biologically independent samples. b, Results of luciferase assays with the 8×GTIIC-Lux reporter in HEK293 cells reconstituted with either YAP(WT) or YAP(WW1mut) and transfected with the indicated siRNAs. Data are normalized to control siRNA-transfected cells and are presented as mean + s.d. of n = 3 biologically independent samples. c, qPCR analyses of the YAP/TAZ targets ANKRD1, CYR61 and PTX3 in MCF10A cells transfected as indicated. Data are mean + s.d. of n = 3 biologically independent samples. d, Western blot analysis of ARID1A, E-cadherin and vimentin from lysates of MCF10A cells transfected with the indicated siRNAs. e, qPCR analyses of CTGF (left) and ARID1B (right) expression in MCF10A cells transfected as indicated. Data are mean + s.d. of n = 3 biologically independent samples. f, Representative confocal images (left) and quantifications (right; >100 cells per condition) of YAP/TAZ localization in MCF10A cells transfected with the indicated siRNAs. g, Western blot analysis of YAP, TAZ and YAP phosphorylated at the key Hippo/LATS target site (p-YAP S127) in lysates of MCF10AT cells transfected with the indicated siRNAs. P values were determined by unpaired two-sided Student’s t-test; n.s., not significant. All panels display representative experiments, repeated independently two (d, e, g) or three (a–c, f) times with similar results.
