Extended Data Fig. 2: Dome response to inhibition of tension and weakening of cell–cell adhesion.
From: Active superelasticity in three-dimensional epithelia of controlled shape
a, Time evolution of surface tension and volume of a representative dome in response to Y27632 (30 μM, added at t = 0 min). b, Cellular areal strain ε c as a function of dome nominal areal strain ε d during dome swelling. Only a subset of cells is represented and most cells with ε c < ε d have been omitted for clarity. Coloured lines represent the cells labelled in c. Dashed line represents the relation ε c = ε d. The inset represents the variance of ε c within the dome as a function of ε d. c, Maximum intensity projection and x–z and y–z confocal sections of an epithelial dome of MDCK–CAAX cells before (−1 min) and after (12 min and 26 min) addition of Y27632 (30 μM, added at t = 0 min). The time evolution of coloured cells is depicted in b using the same colour code. Scale bars, 50 μm. Data are representative of n = 3 experiments. d, Maximum intensity projection and corresponding x–z and y–z profiles, showing the collapse of a dome of MDCK–CAAX cells after treatment with 2 mM EGTA (30 min and 35 min after the addition of EGTA). Data are representative of n = 3 experiments. Scale bar, 50 μm. e, After dome collapse, gaps (red arrowheads) were apparent at tricellular junctions. Scale bar, 10 μm.
