Extended Data Fig. 5: Quality of the density maps in representative regions of the four 5-HT3 receptor reconstructions.
From: Conformational transitions of the serotonin 5-HT3 receptor
Densities in mesh representations overlaid with structures for the T (blue), I1 (yellow), F (purple) and I2 (green) reconstructions and structures, left to right, respectively, in each panel. Views a–e are approximately the same as those in extended data figure 4 of a previous publication of α1 GlyR7. a, Representative densities of the β-sheets in the ECD. b, Densities of the Cys loop and the M2–M3 loop. c, Densities of helices M1 and M2. d, Densities of M3 and M4. e, Densities of M2 at the level of L9′ (L260). f, Densities around the ligands tropisetron (in T) and serotonin (in I2). The 3.2 Å resolution of I2 permits unambiguous orientation of serotonin, and side chains of residues around the ligands are seen in densities. For tropisetron, given the limited 4.5 Å global resolution, the orientation of the ligand and the positions of surrounding side chain are less certain. The views are the same as in Extended Data Fig. 3b, e.
