Extended Data Fig. 3: Cryo-EM map generation, data processing and atomic-model refinement of ABCG2EQ–E1S.
From: Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states
a, Twelve representative 2D class averages of the final round of 2D classification, sorted in decreasing order by the number of particles assigned to each class. b, FSC from the CryoSPARC auto-refine procedure of the unmasked half-maps (blue), the half-maps after masking (green), and the half-maps after masking and correction for the influence of the mask (pink). A horizontal dotted line (blue) is drawn for the FSC = 0.143 criterion. For both the unmasked and the corrected FSC curves, their intersection with the FSC = 0.143 and the FSC = 0.5 lines are marked by arrows, and the resolutions at these points are indicated. c, FSC curve of the final 3.58 Å refined model versus the map against it was refined (FSCfull; black line). The FSC curve of the final refined model with introduced shifts (mean value of 0.3 Å) versus the first of two independent half-maps (half-map 1, against which it was refined; FSCwork; green line) or the same model versus the second independent half-map (against which it was not refined; FSChalf2; red line) is also shown. d, Flow chart for cryo-EM data processing and structure determination of the ABCG2EQ–E1S complex. e, Full view of the final CryoSPARC B-factor-sharpened map of ABCG2EQ–E1S, coloured by local resolution in Å, as calculated by ResMap with the clipping plane in the middle of the molecule. f, Angular distribution plot for the final reconstruction.
