Extended Data Fig. 2: Flash-induced S-state turnover of PSII micro crystals.
From: Structures of the intermediates of Kok’s photosynthetic water oxidation clock
a, Change of the first moment of the in situ-measured Mn Kβ XES as a function of flashes and fit to the data. b, Flash-induced O2 yield as measured by MIMS as a function of flash number and fit to the data. c, The estimated S-state population (%) for each of the flash states from fitting of the XES data and of the flash-induced O2 evolution pattern of a suspension of PSII crystals at pH 6.5. Two different fits were performed: a global fit of both O2 and XES data using an equal miss parameter of 22% and 100% S1 population in the 0F sample (black traces in a, b; S-state distribution listed in the columns headed O2), and a direct fit of the XES data using a 8% miss parameter in the S1→S2 and a 27% miss parameter for the S2→S3 and S3→S0 transitions (XES in c). For the XES fit, shifts of −0.06 eV per oxidation state increase for all S states were assumed. The XES raw spectra are published elsewhere36.
