Extended Data Fig. 3: Performance on NOR task for hippocampal inactivation studies.
From: The hippocampus is crucial for forming non-hippocampal long-term memory during sleep
Memory is indicated by mean ± s.e.m. discrimination ratios during the first 1 min, first 3 min, and entire 5 min of the retrieval phase on the NOR task in experiments involving reversible inactivation of the hippocampus (dot plots overlaid). a, Muscimol (red bars, n = 8 rats, versus vehicle, grey bars, n = 8 rats) was infused into the hippocampus in the post-encoding interval upon the first occurrence of continuous SWS, or 15 min before retrieval testing (blue bars, n = 9 rats). Retrieval was tested 3 weeks after encoding. b, Control study in which muscimol (purple bars, n = 7 rats) was infused shortly after encoding while the rats remained awake during the 2-h post-encoding interval, compared with untreated wake control rats (n = 8 rats, empty bars). Retrieval was tested 1 week after encoding. Infusion of muscimol during post-encoding wakefulness tended to enhance NOR performance, which suggests that during wakefulness hippocampal activity normally interferes with NOR memory consolidation8. It might also reflect compensatory plasticity occurring in extrahippocampal regions upon hippocampal suppression46. c, Control studies in which muscimol (blue bars, versus vehicle, grey bars) was infused 15 min before retrieval testing of recent NOR memory (left, n = 12 rats for each substance condition) or 15 min before the encoding phase (right, n = 6 rats for each substance condition). Retrieval was tested 30 min after encoding, with the rats staying awake during this interval. +++P < 0.001, ++P < 0.01, +P < 0.05 for one-sample t-test against chance level; **P < 0.01, *P < 0.05 for pairwise t-tests (two-sided) between conditions. See Fig. 2 for further details.
