Extended Data Fig. 10: Chronic stress leads to preferential insertion of GluA2-lacking AMPA receptors in D2R-MSNs.
From: Reward behaviour is regulated by the strength of hippocampus–nucleus accumbens synapses
a, Chronic stress does not alter subunit composition in D1R-MSNs. Two-tailed Mann–Whitney U-test of amplitude at +40 mV: U = 35, P = 0.6665, n = 6, 7 mice. b, D2R-MSNs from mice exposed to chronic stress show inward rectification at positive membrane potentials. Two-tailed Mann–Whitney U-test of amplitude at +40 mV: U = 0, P = 0.0006, n = 6, 7 mice. c, NASPM decreases EPSC amplitude in D2R-MSNs from mice exposed to chronic stress. Kruskal–Wallis test: H = 7.423, P = 0.0132, n = 4 mice per group. d, Current–voltage relationships in D1R-MSNs remain unaffected by chronic stress or fluoxetine treatment. Kruskal–Wallis test with Dunn’s post hoc test: H = 0.9436, P = 0.8149, n = 5, 5, 5, 4 mice. e, D2R-MSNs from mice exposed to chronic stress treated with chronic fluoxetine show a linear current–voltage relationship, similar to unstressed controls. Inward rectification is observed in D2R-MSNs from mice exposed to chronic stress alone or chronic stress with acute fluoxetine treatment. Kruskal–Wallis test with Dunn’s post hoc test: H = 31.42, P < 0.0001, n = 5, 5, 5, 8 mice. The line in the middle of the box is plotted at the median. The box extends from the 25th to 75th percentiles. Whiskers represent minimum and maximum. Centre values represent mean and error bars represent s.e.m. ****P < 0.0001, **P < 0.01, *P < 0.05.
