Extended Data Fig. 7: Analysis of defects in the Stella−/− oocyte.
From: Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1
a, A scatter plot showing gene transcriptional changes in StellaΔm/+ and control two-cell-stage embryos. Genes that are upregulated or downregulated more than twofold in StellaΔm/+ embryos are indicated in red and green, respectively. b, ZGA genes are divided into three groups on the bases of their expression changes in StellaΔm/+ two-cell-stage embryos. c, A Venn diagram showing how many of the 167 ZGA-defective genes can also be defined as ZGA-defective (≥twofold decrease) in K/W or W/K spindle-transferred two-cell-stage embryos when compared with W/W two-cell-stage embryos. d, Methylation levels in the promoter regions of ZGA-defective genes defined in the indicated spindle-transferred two-cell-stage embryos. The corresponding methylation level in StellaΔm/+ and control two-cell-stage embryos is shown. e, A Venn diagram showing how many of the 412 upregulation-defective genes can also be defined as upregulation-defective (≥2-fold decrease) in K/W or W/K spindle-transferred two-cell-stage embryos when compared with W/W two-cell-stage embryos. f, Methylation levels in the promoter regions of upregulation-defective genes defined in the indicated spindle-transferred two-cell-stage embryos. The corresponding methylation level in StellaΔm/+ and control two-cell-stage embryos is shown. g, A Venn diagram showing how many of the 102 downregulation-defective genes can also be defined as downregulation-defective (≥2-fold increase) in K/W or W/K spindle transferred two-cell-stage embryos compared with W/W two-cell-stage embryos. h, Methylation levels in the promoter regions of downregulation-defective genes defined in the indicated spindle-transferred two-cell-stage embryos. The corresponding methylation level in StellaΔm/+ and control two-cell-stage embryos is shown. In d, f, h, the midline of the box indicates the median, the outer edges represent the first and third quartiles, and values outside 1.5× IQR are designated outliers and are not shown. The upper and lower whiskers show the maximum and minimum values that are not outliers, respectively. Data were analysed using an unpaired two-tailed Student’s t-test, with three biological replicates for each sample. i, Representative images of the reconstituted embryos analysed in Fig. 3i after 96 h of in vitro culture. W/W, n = 2; K/W, n = 3; W/K, n = 3; K/K, n = 2 independent experiments. Scale bar, 50 μm. j, The preimplantation development rate of the indicated microinjected embryos during in vitro culture. Each dot represents one biological replicate, and the mean values of biological replicates are connected by lines.
