Extended Data Fig. 1: Analysis of regulation between Stella and UHRF1.
From: Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1
a, Stella associated with endogenous UHRF1 in HEK293 cells (left) and NIH3T3 cells (right) under stringent co-immunoprecipitation conditions (extensive wash with 500 mM NaCl). For gel source data, see Supplementary Fig. 1. b, Expression levels (fragments per kilobase of transcript per million, FPKM) of the most highly expressed genes and Uhrf1 in Stella+/− D5, D10, D20 and MII oocytes. c, Analysis of UHRF1 distribution in wild-type D20 growing oocytes; PFA/Triton co-fixation was performed before PFA fixation (see Methods). Left, representative images of the stained oocytes. Right, quantitative results of the UHRF1 signal intensities in the nuclear regions (N), cytoplasmic regions (C) and background (B). n indicates the number of oocytes analysed from one representative experiment. d, e, Left, representative images of zygotes (d) and early two-cell-stage embryos (e) stained with anti-Stella (red) and anti-UHRF1 (H-8, green). Right, scatter plots of the quantitative results of the UHRF1 nuclear/cytoplasmic intensity ratio. Het denotes heterozygous Stella+/−; KO denotes knockout Stella−/−. n indicates the number of cells analysed from two independent experiments. In c–e, data are mean ± s.d. and are analysed using an unpaired two-tailed Student’s t-test. ns, not significant (P > 0.05). In a, c, data shown are representatives of three independent experiments. Scale bars, 10 μm.
