Extended Data Fig. 4: Generation and analysis of the Uhrf1 conditional knockout mouse.
From: Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1
a, An illustration of the targeted Uhrf1 allele: Uhrf1tm1a(EUCOMM)Wtsi and the strategy to generate the Uhrf1 conditional knockout mouse. b, Immunostaining of wild-type and Uhrf1−/− fully grown oocytes with anti-UHRF1 antibodies (H-8 or M-132). The experiment was performed once with four independent anti-UHRF1 antibodies. Scale bars, 10 µm. c, Representative images of preimplantation embryos after 96 h of in vitro culture. Stella+/−, n = 3; Stella−/−, n = 2; Stella+/−Uhrf1−/−, n = 2; Stella−/−Uhrf1−/−, n = 2 independent experiments. Scale bar, 100 μm.
