Extended Data Fig. 6: Functional roles of FBXO38 in MC38 colorectal carcinoma model.
From: FBXO38 mediates PD-1 ubiquitination and regulates anti-tumour immunity of T cells
a, Gating strategy of tumour-infiltrating leukocytes. Cells were first gated by FSC/SSC to exclude debris, followed by gating FSC-A and FSC-H to eliminate non-singlet cells. CD8+ cells were gated for CD44 and PD-1 expression analysis. Conventional CD4+ (CD25−) cells and Treg cells (CD4+FOXP3+) were gated for PD-1 expression. This gating strategy is applicable to c–g and Fig. 3e–o. b–g, MC38 tumour progression and phenotype of tumour-infiltrating T cells were assessed in wild-type and Fbxo38CKO mice. b, Tumour size was recorded every two days from day 7. n = 10 mice. c–g, Tumour-infiltrating T cells were isolated and analysed on day 16. n = 6 mice. PD-1 levels of CD8+, conventional CD4 (CD4+CD25−) and Treg (CD4+FOXP3+) cells were assessed in c–e. Total number of CD8+ T cells, total number of CD4+ T cells, the CD8+/CD4+ cell ratio and Treg cell population in the tumour microenvironment were also assessed in f, g. b–g, Data are representative of two independent experiments. **P < 0.01, unpaired Student’s t-test (c, d (right), e, f (left, middle)), unpaired Student’s t-test with Welch’s correction (d (left), f (right), g) or two-way ANOVA (b).
