Extended Data Fig. 7: Fbxo38 knockdown does not affect CD8+ T cell activation.
From: FBXO38 mediates PD-1 ubiquitination and regulates anti-tumour immunity of T cells
a, b, Fbxo38 knockdown efficiency. c, TCR and CD28 surface levels of control and Fbxo38-knockdown cells. d–g, Control and Fbxo38-knockdown CD8+ T cells were sorted and stimulated with anti-CD3/CD28 to measure TCR signalling and cytokine production. d, Phosphorylation of TCR signalling proteins in control and Fbxo38-knockdown CD8+ T cells after stimulation for indicated times with soluble anti-CD3/CD28 (5 μg ml−1). e, CD69 surface levels in control and Fbxo38-knockdown CD8+ T cells after stimulation for 5 h with anti-CD3/CD28 (5 μg ml−1). n = 3 biologically independent samples. f, g, IFNγ secretion in control and Fbxo38-knockdown CD8+ T cells after stimulation for 24 h (f) or 48 h (g) with anti-CD3/CD28 (5 μg ml−1). n = 3 biologically independent samples. a–g, Data are representative of at least three independent experiments. e–g, Data were analysed by unpaired Student’s t-test. For gel source data, see Supplementary Fig. 1.
