Extended Data Fig. 5: Regulation of PD-1 surface levels by FBXO38.
From: FBXO38 mediates PD-1 ubiquitination and regulates anti-tumour immunity of T cells
a, b, PD-1 surface levels in activated CD8+ T cells of Fbxo38flox/flox (wild-type) or Fbxo38CKO (CKO) mice at different time points. Naive CD8+ T cells were stimulated with anti-CD3/CD28 (2 μg ml−1 or 5 μg ml−1). n = 3 biologically independent samples. c–f, Assessment of the role of FBXO38 in p97-mediated degradation of PD-1 and TCR at the endoplasmic reticulum. c, d, Naive CD8+ T cells from wild-type or Fbxo38CKO mice were stimulated with anti-CD3/CD28 (2 μg ml−1) for 96 h and incubated with the p97 inhibitor CB-5083 (0.5 or 1 μM) for 12 h. n = 3 biologically independent samples. e, f, Naive CD8+ T cells from wild-type or Fbxo38CKO mice were incubated with the p97 inhibitor CB-5083 (0.5 or 1 μM) for 12 h. n = 3 biologically independent samples. Data are representative of two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired Student’s t-test (a–c, e, f) or unpaired Student’s t-test with Welch’s correction (d).
