Extended Data Fig. 6: In vitro translation activity of O-stapled ribosome variants upon inhibition of native ribosome subunits.
From: Controlling orthogonal ribosome subunit interactions enables evolution of new function
This figure uses the same dataset as in Fig. 2g, and shows the in vitro translation of T7-O-GFP in S30 extracts containing different O-stapled ribosome variants in the presence of 10 μM spectinomycin and 50 μM erythromycin (which inhibit contributions to translation from endogenous subunits). The dots indicate individual data for each tested O-stapled ribosome, and the grey bars show means, for the number of independent replicates given in the Methods. O-d2d8 is highlighted in blue, and the error bars indicate ± s.d.
