Extended Data Fig. 5: Activities of O-stapled-ribosome linker-length variants in cell-free protein synthesis.

a, Activities of lysates that contain a mixture of host and O-stapled ribosomes on a WT-GFP reporter, showing that the translation machinery in the tested lysates is equally active. Every data point shows the activity of an independently produced S30 extract from independently grown cells. b, Activities of the same set of lysates as in a on an O-GFP reporter, normalized to their activity on the WT-GFP reporter. Every data point shows the activity of an independently produced S30 extract from independently grown cells. Detailed statistics are shown in Supplementary Data 3. c, The same dataset as in b, represented as a heatmap for ease of comparison. d, Activity of lysates containing a mixture of host and O-stapled ribosomes on the WT-GFP reporter. Every data point shows independently measured activities of O-stapled ribosome variants in independently prepared S30 extracts. e, Activity of the same set of the lysates as in d on the O-GFP reporter in the presence of 10 μM spectinomycin and 50 μM erythromycin, normalized to their activity on WT-GFP. Data points are independently measured activities of O-stapled ribosome variants in independently prepared S30 extracts. Detailed statistics are given in Supplementary Data 4. f, The same dataset as in e, represented as a heatmap for ease of comparison. In a, b, d and e, the error bars show ± s.d., the grey bar shows the mean of the number (n) of independent replicates. Values for n are given in the Methods.