Extended Data Fig. 8: Comparison of conformations of different structures of monomeric gp120 and various V3 loops.

a, Comparison of structures of an unliganded gp120 core (PDB ID:4OLV; purple), a CD4-bound monomeric gp120 core with the V3 loop (PDB ID: 2QAD; blue) and gp120 in complex with CD4 and CCR5 from this study (cyan). The gp120 core region is marked by a circle with a diameter of 50 Å. The N and C termini, V1V2 stem, V3 stem or loop and bridging sheet are indicated. b, Representative conformations that an HIV-1 V3 loop can adopt. From left to right, V3 loop in the unliganded SOSIP BG505 Env trimer (PDB ID: 4ZMJ); the first-V3-containing gp120 core in complex with CD4 and antibody X5 (PDB ID: 2B4C²⁹); CD4- and 412d-bound monomeric gp120 core with V3 (PDB ID: 2QAD); CCR5-bound intact gp120 (this study); and V3 peptide in complex with antibody 447-52D (PDB ID: 3GHB³⁶); antibody 268-D (PDB ID: 3GO1³⁷); antibody 2557 (PDB ID: 3MLV³⁷); and antibody 10A37 (PDB ID: 5V6L³⁸). The root-mean-square deviation of each structure (except for 5V6L), relative to the CCR5-bound gp120 monomer, is shown at the bottom in parentheses.