Extended Data Fig. 8: Generation of TRM cells by localized deposition, and antibody strategies for selective TCIRC or TRM depletion.
From: Tissue-resident memory CD8+ T cells promote melanoma–immune equilibrium in skin
WT mice received activated gBT-I.CD45.1 or gBT-I.Thy1.1 cells (1 × 106 or 4 × 106) by e.c. transfer. a, Frequency and phenotype of transferred Vα2+ gBT-I.CD45.1 cells in the spleen and skin at 8 days and more than 4 weeks after transfer. Data representative of n = 2 biologically independent experiments with n = 10 mice (day 8) and n = 8 mice (more than 4 weeks). b, Number of gBT-I.CD45.1 cells isolated from spleen and skin more than 4 weeks after transfer, determined by flow cytometry. Data pooled from n = 5 biologically independent experiments with n = 21 mice. c, d, Frequency (c) and number (d) of e.c. transferred gBT-I.Thy1.1 cells isolated from the indicated organs more than 7 days after intraperitoneal (i.p.) treatment with PBS (‘Control’) or anti-Thy1.1 antibody (‘Depletion’). Data representative of (c) or pooled from (d) n = 2 biologically independent experiments with n = 9 mice per group. ***P = 0.0002, Mann–Whitney test. e, f, Frequency (e) and number (f) of e.c. transferred gBT-I.Thy1.1 cells in the spleen and skin more than 50 days after i.p. treatment with low-dose anti-Thy1.1 antibody and intradermal (i.d.) treatment with high-dose anti-Thy1.1 antibody or PBS. Data representative of (e) or pooled from (f) n = 4 biologically independent experiments with n = 20 (i.p. only) or n = 22 (i.p. + i.d.) mice. Only values greater than zero are plotted on the logarithmic scale. *P = 0.0261, Mann–Whitney test.
