Extended Data Fig. 1: Growth kinetics and persistence of B16.gB cells after e.c. inoculation.
From: Tissue-resident memory CD8+ T cells promote melanoma–immune equilibrium in skin
a, Rate of tumour incidence across individual experiments after s.c. (Subcut.) and e.c. (Epicut.) inoculation with either B16.gB or B16.gB.Luc cells in WT mice. Data pooled from n = 3 (s.c.) and n = 46 (e.c.) biologically independent experiments with n = 15 (s.c.) or n = 452 (e.c.) mice. b, Tumour growth kinetics after s.c. or e.c. inoculation with B16.gB or B16.gB.Luc cells. Data pooled from n = 3 biologically independent experiments with n = 15 mice (s.c.) or n = 22 independent experiments with n = 154 mice (e.c.). c, Photographs of metastases in tumour-draining brachial lymph nodes (LNs) of mice with progressive melanoma following e.c. B16.gB inoculation. Representative of n = 46 biologically independent experiments. d, B6 albino mice were inoculated epicutaneously with B16.gB.Luc cells and subjected to longitudinal bioluminescence imaging. Bioluminescence signals (arrows) emitted from progressing melanomas or from skin of non-developer mice. Data representative of n = 2 biologically independent experiments with n = 18 mice. e, Two-photon microscopy image of a macroscopic melanoma 2 or more weeks after e.c. inoculation with B16.Tyr–/–.mCherry cells. f, Two-photon microscopy images of macroscopically tumour-free skin with persistent B16.Tyr–/–.mCherry cells (arrows) at indicated times post-inoculation. e, f, B16.Tyr–/–.mCherry (B16) cells, red; SHG, blue. Note autofluorescent hair (green). Data representative of n = 3 biologically independent experiments with n = 22 mice.
