Extended Data Fig. 3: Generation of melanoma-associated TRM cells does not require local antigen.
From: Tissue-resident memory CD8+ T cells promote melanoma–immune equilibrium in skin
a, Experimental protocol. WT mice were inoculated epicutaneously with B16.gB cells on the upper left flank and monitored for tumour progression. Mice developing macroscopic melanoma were co-transferred intravenously (i.v.) with equal numbers of naive gBT-I.CD45.1 cells and OT-I.CD45.1.CD45.2 cells, and challenged with HSV.Ova on the lower right flank. Tissues were harvested for fluorescence-activated cell sorting (FACS) analysis 4 weeks after HSV infection. b, Surface phenotype of gBT-I or OT-I cells isolated from peritumoural skin 4 weeks after HSV infection. c, d, Proportion of gBT-I or OT-I cells expressing CD69 and CD103 (c) or CD69 only (d) in the indicated organs (Spl, spleen; Peritum, peritumoural). Data are representative of (b) or pooled (c) from one experiment with n = 6 mice.
