Extended Data Fig. 5: MHC I expression by progressing or controlled melanoma.
From: Tissue-resident memory CD8+ T cells promote melanoma–immune equilibrium in skin
a, WT mice were transferred naive gBT-I.CD45.1 cells and inoculated epicutaneously with B16.gB.Tyr–/–.mCherry cells. Tumours isolated from mice with progressively growing melanomas (defined by an increase in tumour volume over at least four consecutive days; ‘escaped’) or tumours that remained small and did not progress (defined by a stable tumour volume over a period of 1.5–3 weeks; ‘controlled’) were harvested and analysed by flow cytometry. a, Proportion of B16.gB.Tyr–/–.mCherry cells isolated from escaped or controlled tumours that expressed MHC class I (H2-Kb) proteins at the time of harvest. ***P = 0.0003. b, Geometric mean fluorescence intensity (gMFI) of MHC I expression by B16.gB.Tyr–/–.mCherry cells isolated from escaped or controlled tumours, normalized to the gMFI of fluorescence minus one (FMO) controls. ***P = 0.0005 Data in a, b pooled from n = 3 biologically independent experiments with n = 19 (escaped) or n = 8 (controlled) mice. Mann–Whitney test.
