Extended Data Fig. 6: Brain T_reg cells proliferate in response to IL-33.

a, The relative expression levels of Il33 in the ischaemic brains at the indicated time points after stroke onset were evaluated by qPCR (n = 6 day 0, n = 3 others). Immunohistochemical staining for IL-33 in the brains of Il33^−/−, wild-type sham or wild-type ischaemic mice on day 14 after stroke onset. Data are representative of two independent experiments. b, Flow cytometric analysis of ST2 in the ischaemic brains of wild-type, Il33^−/−, or Il1rl1^−/− mice on day 14 after stroke onset. The frequency of ST2⁺ cells of T_reg cells was determined (n = 3). Data are representative of two independent experiments. c, Flow cytometric analysis of the frequency of T_reg cells among CD4⁺ T cells from the ischaemic mice administrated with anti-ST2 antibody on days 7, 10 and 12, and analysed on day 14 after stroke onset. The frequency of T_reg cells of CD4⁺ T cells was determined (n = 5 control IgG-treated mice, n = 4 ST2 antibody-treated mice). Data are representative of a single experiment. d, Flow cytometric analysis of the frequency of ST2⁺ cells among brain cells from the ischaemic mice on day 14 after stroke onset. (n = 4). Data are representative of two independent experiments. e, T_reg cells (1 × 10⁶) from spleens and lymph nodes from wild-type or Il1rl1^−/− mice were transferred into Rag2^−/− mice on day 5 after stroke onset. The representative plots of CD4⁺ cells from the indicated tissues in the ischaemic brains on day 14. Data are representative of two independent experiments and shown as mean ± s.e.m. P values determined by two-tailed Student’s t-test.

Source Data