Extended Data Fig. 7: Expression of chemokine receptors on CD4+ T cells.
From: Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery
a, TH1 (IFNγ+), IFNγ−FOXP3− and Treg (FOXP3+) cells were isolated from the day-14 post-ischaemic brains of 8 mice and the relative expression levels of the indicated chemokine receptors on CD4+ T cells were evaluated by qPCR. Data were obtained from pooled samples of eight mice, and representative of two independent experiments. b, Oligodendrocytes, astrocytes and microglia were isolated from ischaemic brains at the indicated time points after stroke onset and the relative expression of Ccl1 and Ccl20 was evaluated by qPCR (n = 3 sham and day-14 mice, n = 2 day 3 and 7 mice). Data are representative of two independent experiments. c, Representative plots of flow cytometric analysis of CD4+ cells and the absolute number of Treg cells from the brains of wild-type or Ccr6−/− mice in Fig. 3h at day 14 after stroke onset (n = 6 wild-type mice, n = 4 Ccr6−/− mice). Data are representative of two independent experiments. d, Representative plots of flow cytometric analysis of CD4+ cells and the absolute number of Treg cells from day-14 post-ischaemic mice injected intraventricularly with CCL1 or CCL20 proteins on days 6 and 9 post-stroke-onset in Fig. 3i (n = 6 PBS-treated mice, n = 5 CCL1-treated mice, n = 6 CCL20-treated mice). Data are representative of two independent experiments. P values determined by two-tailed Student’s t-test. Data are mean ± s.e.m.
