Extended Data Fig. 10: Brain slice and gating strategy of flow cytometric analysis.
From: Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery
a, For evaluation of ischaemic brain tissues, 1-mm-thick serial coronal slices from the brain were embedded in paraffin. The infarct area was detected by MAP2 staining of all slices (slices 1–6). The reactive astrocyte area (astrogliosis area) was detected by GFAP staining of slice 3 at the ischaemic core of the transient middle artery occlusion. b, Gating strategy of analysis of various brain cells. c, A scheme of regulation of brain damage by brain Treg cells.
