Extended Data Fig. 9: GC-specific mTOR localization.
From: Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex
a, High-magnification views of representative GCs from callosal projection neurons immunolabelled for mTOR, equivalent to Fig. 5c and Extended Data Fig. 8b mTOR panels, but with a distinct mTOR antibody to independently confirm dense focal mTOR in GCs. Top, overlay images; bottom, heat maps of the same GCs. b, Example of mTOR labelling (red in two left panels; heat map in right panel) in 3-day-cultured neurons. A GFP-labelled axon from an electroporated inter-hemispheric neuron displays dense focal mTOR in its GC (arrow) compared to adjacent cell bodies (asterisks; DNA in blue indicates nuclei). Two other unlabelled GCs in the field can be recognized by virtue of their dense focal mTOR labelling alone (arrowheads). c, Example of dendritic GCs (arrows) lacking mTOR, juxtaposed to an unlabelled GC (arrowhead) with prominent mTOR focus. Bars in a–c (bottom right of each) indicate mTOR intensity heat-map colour range, as well as 10-μm scale. GCs imaged from n = 2 biological replicates from independent in utero electroporations.
