Extended Data Fig. 1: Knock-in strategy using the TyrCopyCat-targeting vector.
From: Super-Mendelian inheritance mediated by CRISPR–Cas9 in the female mouse germline
The U6-Tyr4a gRNA (tyrosinase exon 4 gRNA a) and CMV-mCherry were inserted into the cut site of the Tyr4a gRNA by HDR after CRISPR–Cas9 DSB formation targeted by the Tyr4a gRNA. See Supplementary Methods and Supplementary Figs. 1, 2 for additional details.
