Extended Data Fig. 7: Characterization of IFNγ+CD8 TILs induced by the 11-mix.
From: A defined commensal consortium elicits CD8 T cells and anti-cancer immunity
a, Experimental design for germ-free mouse-based studies in b and c (and Fig. 4a–c). C57BL/6 germ-free mice were colonized with 11- or 10-mix on day −7, or left uncolonized, and were subjected to subcutaneous implantation of MC38 adenocarcinoma cells on day 0. Anti-PD-1 monoclonal antibody was injected intraperitoneally every third day between days 3 and 9. b, Representative flow cytometry histograms showing the expression of CD103, PD-1, ICOS, CD44 and KLRG1 by colonic lamina propria or TIL IFNγ+ CD8 T cells from the indicated mice. c, Left, Frequencies of TCR Vβ gene usage among IFNγ+ CD8 TILs from GF+MC38+anti-PD-1 (grey) or GF+11-mix+MC38+anti-PD-1 (red) mice. Right, Frequencies of TCR Vβ gene usage among IFNγ+ CD8 T cells from the colons of GF+11-mix mice (blue) or the tumours of GF+11-mix+MC38+anti-PD-1 (red). Each circle represents an individual mouse (TIL) or a pool of mice (colon). The number of mice in each group is shown. Data are mean and s.d. **P < 0.01; *P < 0.05; two-tailed unpaired t-test. See Source Data for exact values.
