Extended Data Fig. 10: Antigen-specificity and TCR Vβ usage of IFNγ+CD8 T cells induced by the 11-mix differ by anatomical location.
From: A defined commensal consortium elicits CD8 T cells and anti-cancer immunity
a, Percentage of IFNγ+ cells among CD8 T cells in either colons from GF+11-mix mice or MC38 tumours from SPF+11mix+anti-PD-1 mice, after ex vivo stimulation with the indicated antigens. b, Principal component analysis plots of TCR Vβ usage by the IFNγ+ and IFNγ- CD8 T cell subsets isolated from the indicated tissues of GF+11-mix (right) or germ-free (left) mice. For TIL data, IFNγ+ and IFNγ− CD8 T cells were isolated from germ-free ± 11mix+MC38+anti-PD-1 mice. Two-way ANOVA interaction P values comparing two populations at a time are as follows. GF group: 0.006 (CLP IFNγ+ versus iLN IFNγ+), 0.50 (CLP IFNγ+ versus TIL IFNγ+), 0.20 (iLN IFNγ+ versus TIL IFNγ+), <0.0001 (CLP IFNγ− versus iLN IFNγ−), <0.0001 (CLP IFNγ− versus TIL IFNγ−), 0.0024 (iLN IFNγ− versus TIL IFNγ−). GF+11-mix group: <0.0001 (CLP IFNγ+ versus iLN IFNγ+), <0.0001 (CLP IFNγ+ versus TIL IFNγ+), 0.0001 (iLN IFNγ+ versus TIL IFNγ+), <0.0001 (CLP IFNγ− versus iLN IFNγ−), <0.0001 (CLP IFNγ− versus TIL IFNγ−), 0.0009 (iLN IFNγ− versus TIL IFNγ−). Each circle represents an individual mouse (iLN and TIL) or a pool of mice (colon). The number of mice in each group is shown. Data are mean and s.d. ***P < 0.001; **P < 0.01; one-way ANOVA with Tukey’s test. See Source Data for exact P values.
