Extended Data Fig. 1: Characterization of the intestinal IFNγ⁺ CD8 T cell population.

a, b, Representative flow cytometry histograms and plots showing the expression of CD8β, CD44, T-bet, ICOS, CD103, KLRG1 and GrB by colonic lamina propria IFNγ⁺ CD8 T cells isolated from SPF mice from two independent experiments. c, Number of IFNγ⁺ CD8 T cells in the colonic or small intestinal lamina propria of germ-free and SPF mice. d, Percentage of IFNγ⁺ cells among CD8 T cells in the colon of SPF mice (between 8 and 12 weeks old) treated with or without AVMN (ampicillin, vancomycin, metronidazole and neomycin) via the drinking water for 4 weeks. e, Faeces from SPF mice (SPFfae) were orally inoculated into germ-free mice (7 weeks old), and 4 weeks later the percentage of IFNγ⁺ cells among CD8 T cells was analysed. f, Left, percentage of IFNγ⁺ cells among CD8 T cells in the colonic lamina propria of SPF C57BL/6 mice (10 weeks old), reared at the indicated institute or vendor. Right, SPF C57BL/6 mice (8 weeks old) obtained from Charles River were cohoused with C57BL/6 mice from CLEA for 0, 2 or 6 weeks, and the percentage of IFNγ⁺ cells among colonic lamina propria CD8 T cells was analysed. Each circle represents an individual animal, and the number of mice in each group is shown. Data are mean and s.d. ***P < 0.001; *P < 0.05; one-way ANOVA with Tukey’s test (c, f) or two-tailed unpaired t-test (d, e). See Source Data for exact P values.

Source Data