Extended Data Fig. 3: Induction of IFNγ+CD8 T cells by the 11-mix via non-inflammatory immunomodulation.
From: A defined commensal consortium elicits CD8 T cells and anti-cancer immunity
a, Percentage of IFNγ+ cells among colonic CD8 T cells from mice of the indicated genetic background, colonized with or without the 11-mix. b, c, Representative flow cytometry histograms and plots showing the expression of CD103, PD-1, CD44, T-bet, ICOS, KLRG1 and GrB by colonic IFNγ+ CD8 T cells from germ-free mice colonized with or without the 11-mix for 4 weeks, from two independent experiments. d, Expression of the indicated pro-inflammatory genes in colonic epithelial cells from germ-free, GF+11-mix (4 weeks after colonization), and SPF mice, as determined by qPCR. e, Representative photographs of caecums and colons from germ-free, GF+11-mix (4 weeks after colonization) and SPF mice from several independent experiments. f–h, Haematoxylin and eosin-stained colon photomicrographs (f), histology score (g), and percentage of IFNγ+ cells among CD8 T cells in the colonic lamina propria (h) of germ-free and GF+11-mix mice (4 weeks or 6 months after colonization). Scale bar, 50 μm. i, Percentages of IL-17A+ (TH17) and IFNγ+ (TH1) cells among CD3+TCRβ+CD4 T cells and IFNγ+ cells among CD3+TCRβ+CD4− T cells (representing primarily CD8 T cells) in the colons of gnotobiotic mice colonized with the 11 strains, a reported TH1 cell-inducing Klebsiella pneumoniae strain (Kp2H7), or 20 reported TH17 cell-inducing strains (TH17 20-mix). Each circle represents an individual animal. The number of mice in each group is shown. Data are mean and s.d. ***P < 0.001; **P < 0.01; *P < 0.05; two-tailed unpaired t-test (a) or one-way ANOVA with Tukey’s test (d, g, h, i) was used. See Source Data for exact P values.
