Extended Data Fig. 4: Cryo-EM active-state processing and map resolution calculations.
From: Structural insights into the activation of metabotropic glutamate receptors
a, Flow chart indicating the processing of cryo-EM data for mGlu5 in complex with Nb43 and l-quisqualate. b, Three-dimensional density maps of mGlu5 (left) and mGlu5 in complex with Nb43 and l-quisqualate (right), coloured by local resolution. c, ‘Gold standard’ FSC curves from RELION indicate that the maps for both mGlu5 and mGlu5 in complex with Nb43 and l-quisqualate reach nominal resolutions of 4.0 Å at FSC = 0.143.
