Extended Data Fig. 9: Structural transitions in the 7TM domain upon activation.
From: Structural insights into the activation of metabotropic glutamate receptors
a, b, Activation involves a 20 Å translation of the 7TM domains relative to each other (a) followed by a 20° rotation around TM4 (b). The 7TM domains of the apo state are shown as shades of purple, whereas the active state 7TM domains are shown as shades of blue and teal. c, Model of the I7916.56C mutation on the active-state structure shows its ideal positioning for disulfide formation. d, Western blot analysis of disulfide formation involving I7916.56C in both wild type and the C129A background in the presence and absence of the NAM MPEP. Bars in d (bottom) represent mean ± s.e.m. from 8 independent experiments. Statistics were performed using repeated measures one-way ANOVA followed by Tukey’s multiple comparisons test and indicated P values were adjusted to account for multiple comparisons.
