Extended Data Fig. 8: Evolution of the glioma genome.

a, Disease course of patient 28 (GBM (IDH WT)) who received treatment with concurrent RT/TMZ, bevacizumab, and a PD-1 inhibitor. The patient underwent three tumour resections and one CSF collection and all four biospecimens were sequenced. The CDK4 amplification was seen in all four samples. Amplifications of PDGFRA/KIT were observed in tumour sample 3, whereas the later CSF sample (4) no longer showed the PDGFRA/KIT amplification. b, Disease course of patient 7 (IDH mutant anaplastic astrocytoma). The patient underwent four tumour resections and two CSF collections. All six samples were profiled. MRIs (T1 post-contrast) correspond to the time of each tissue resection or CSF recollection. Bottom, heat map shows all mutations across the six samples. Diamond, tumour samples profiled; circle, CSF samples profiled. Heat map indicates the variant allelic frequency of the indicated SNVs.