Extended Data Fig. 2: Cells in crisis show characteristics of autophagy and not apoptosis.
From: Autophagic cell death restricts chromosomal instability during replicative crisis
a, Cells at the indicated population doublings were stained with annexin V and PI and the percentages of double-positive cells were measured by flow cytometry. Scatter plot with bars showing the percentage of dying cells approaching crisis. Bars represent mean ± s.d. n indicates number of samples analysed. One experiment was performed. One-way ANOVA; ns, not significant, ∗∗∗P < 0.001. b, Representative phase contrast and fluorescence images of growing, crisis, and staurosporine-treated growing cells (1 μM for 6 h). DAPI staining in blue. One experiment was performed. Scale bar, 50 μm. c, Representative electron micrographs of autophagy-related structures present in crisis fibroblasts and epithelial cells. The images show double-membraned autophagosomes and simple-membraned autolysosomes with cargo at different stages of digestion. Two experiments were performed. d, Box and whisker plots showing the number of autophagic vacuoles per field of view. Centre line, median; box limits, first and third quartiles; whiskers, minimum and maximum. n shows number of images analysed. One-way ANOVA; ∗∗∗P < 0.001. AVs, autophagic vacuoles. For gel source data, see Supplementary Fig. 1.
