Extended Data Fig. 7: ChIP–PCR validation of FOXK1 and FOXK2 target genes.

a–i, Schematic of predicted binding sites and real-time PCR results for FOXK1 and FOXK2 target genes: Hk2 (a), Pfkm (b), Aldoa (c), Pkm (d), Mct1 (e), Pdk1 (f), Pdk4 (g), Pdp1 (h), and Glud1 (i). Mct1 site #2 is not a FOXK1/FOXK2 binding site and serves as a negative control. Input and ChIP cycle threshold (Ct) values were normalized separately to empty vector control as 1. a, Hk2 ChIP–PCR, n = 2 for control input and FOXK2 ChIP, n = 3 for others; b, Pfkm ChIP-PCR, n = 2 for control input, n = 3 for others; c, Aldoa ChIP–PCR, sites #1 and #3, n = 2 for FOXK1 ChIP, n = 3 for others, site #2, n = 3; d, Pkm ChIP–PCR, n = 2 for control input, n = 3 for others; e, Mct1 ChIP–PCR, site #1, n = 2 for control input, n = 3 for others, site #2, n = 2 for ChIPs, n = 3 for inputs; f, Pdk1 ChIP–PCR, n = 2 for FOXK1 ChIP, n = 3 for others; g, Pdk4 ChIP–PCR, site #1, n = 2 for control and FOXK2 ChIP, n = 3 for others, site #2, n = 2 for control ChIP, n = 3 for others; h, Pdp1 ChIP–PCR, n = 2 for ChIPs, n = 3 for inputs; i, Glud1 ChIP-PCR, n = 3. Experiments replicated once. Unpaired two-sided Student’s t-test, ***P < 0.001, **P < 0.01, *P < 0.05.