Extended Data Fig. 5: FOXK1 and FOXK2 regulate proteins involved in the glycolytic pathway. | Nature

Extended Data Fig. 5: FOXK1 and FOXK2 regulate proteins involved in the glycolytic pathway.

From: FOXK1 and FOXK2 regulate aerobic glycolysis

Extended Data Fig. 5

a–k, Western blot experiments for proteins involved in the glycolytic pathway in 3T3‐L1 adipocytes overexpressing or knocked down for FOXK1 or FOXK2. a, HK2; b, PFKM; c, PKM‐M2; d, ALDOA; e, LDHA; f, MCT1; g, PDK1; h, PDK4; i, PDP1; j, GLUD1. k, Phosphorylation of Ser293, Ser232, and Ser300 of the E1α regulatory subunit using specific antisera. Lower parts show PDC complex after immunoprecipitation by PDH Immunocapture Kit and silver staining. Representative experiments from independent replicate samples are shown. l, Schematic view of FOXK1 and FOXK2 regulation sites in the glycolytic pathway. For gel source data, see Supplementary Fig. 1.

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