Extended Data Fig. 9: Purification and substrate cleavage of CasX with a NTSB deletion.
From: CasX enzymes comprise a distinct family of RNA-guided genome editors
a, The representative S200 size-exclusion traces by absorbance at 280 nm for wild-type CasX and for CasX with a deletion of the NTSB domain. SDS–PAGE of wild-type CasX protein and CasX protein with a deletion of the NTSB domain by Coomassie brilliant blue staining is shown in the right panel. b, Comparison of the cleavage activities of wild-type CasX and CasX with a truncation of the NTSB domain on an unwound probe (only the PAM region is base-paired, the rest of the probe is mismatched) and on just a single target DNA strand. All the assays have been repeated three times with consistent results.
