Extended Data Fig. 4: Electron microscopy analysis of CasX–guide RNA–DNA ternary complex with full R-loop (45-bp target DNA).
From: CasX enzymes comprise a distinct family of RNA-guided genome editors
a, Target DNA sequence in this ternary complex. b, Cryo-EM analysis pipeline. From 5,000 drift-corrected micrographs, 1,135,443 particles were picked and used for 2D classification. By 2D-based manual screening, 485,163 good particles were selected for 3D classification into 4 classes. From the class showing better structure preservation, 222,927 particles were further used for heterogeneous refinement; this generated two models, state I and state II, which contained 67% and 33% of the particles, respectively. State I and state II were then independently refined to 3.2 Å and 5.2 Å, respectively. c, The Euler angle distribution for state I and state II. d, GSFSC curve calculated using two independent half-maps. e, Cryo-EM structures of state I and state II coloured by local resolution as calculated in Cryopsarc. The resolution ranges from 3 Å to 7 Å. c and d are standard outputs of Cryosparc.
