Extended Data Fig. 8: Co-culture of cancer cells and neutrophils does not lead to the accumulation of key mutational events.
From: Neutrophils escort circulating tumour cells to enable cell cycle progression
a, Schematic of the experimental design. Neutrophils were purified from healthy donor blood and cultured with either CTC-derived cell lines (BR16, Brx50) or LM2 cells for 72 h. Tumour cells were collected, and isolated gDNA was processed for WES. b, Tile plot showing the mutation status of all key loci found mutated in patients with CTC–neutrophil clusters. None of the CTC–neutrophil-cluster-associated mutations were detected upon co-culture of cancer cells with neutrophils.
