Extended Data Fig. 10: Expression of cell-adhesion molecules–receptor pairs on CTC–neutrophil clusters.
From: Neutrophils escort circulating tumour cells to enable cell cycle progression
a, Schematic of the experimental design (top). The heat map shows the expression landscape of cell-adhesion molecules (CAMs) and corresponding receptors that are expressed in at least 20% of CTC–neutrophil clusters (bottom). The CAM–receptor pairs that are expressed in at least 50% of CTC–neutrophil clusters are shown in red. b, Schematic of the experiment (top). The heatmap shows the expression landscape of CAM receptors and corresponding CAMs that are expressed in at least 20% of CTC–neutrophil clusters (bottom). The CAM–receptor pairs that are expressed in at least 50% of CTC–neutrophil clusters are shown in red. c, Tumour growth curves representing mean tumour volume measurements of mice injected with 4T1-Cas9-GFP cells expressing a control vector (CTRL sgRNA) or sgRNA pools targeting F11r, Icam1, Itgb2 and Vcam1 (CRISPR pool). d, Plot showing the proportion of reads derived from sgRNAs targeting F11r, Icam1, Itgb2 and Vcam1 (4 sgRNAs each) in the 4T1-Cas9-GFP cell line upon library transduction as well as in three primary tumours from NSG-4T1-Cas9-GFP mice. All sgRNAs were represented in the tumour until the end of the experiment. e, Tumour growth curves representing mean tumour volume measurements of mice injected with 4T1-Cas9-GFP cells expressing a control vector (CTRL sgRNA) or individual sgRNAs targeting Vcam1. n = 3; error bars, s.e.m.; ns, not significant by two-sided Student’s t-test (c, e).
