Extended Data Fig. 1: CTC capture in patients with breast cancer and in mouse models.
From: Neutrophils escort circulating tumour cells to enable cell cycle progression
a, Schematic of the CTC capture strategy with the Parsortix device. b, Schematic of the experimental design. Fifty single CTCs and fifty CTC clusters are spiked into blood to assess capture rate. c, Representative images of CTCs and WBCs captured on the Parsortix device and stained for EpCAM, HER2, EGFR (green) and CD45 (red) (left). Plot showing the mean CTC capture efficiency (right). d, Schematic of the experimental design. Fifty single CTCs are spiked into blood to evaluate artificial CTC aggregation rate during processing. e, Representative image of captured CTCs (left). The plot shows the mean per cent of captured single CTCs, CTC clusters and CTC–WBC clusters (right). n = 3; error bars, s.e.m. (c–e). f, Representative images of CTCs from patients and mouse models. n = 34 for patients, n = 8 for NSG-LM2-GFP and NSG-4T1-GFP, n = 7 for BALB/c-4T1-GFP, n = 5 for MMTV-PyMT. g, Plot showing mean CTC counts in patients. n = 34; error bars, s.e.m. h, Plot showing the number of CTCs in each patient-derived CTC–WBC cluster. The red line represents the mean. i, Plot showing mean CTC counts in mouse models. n = 8 for NSG-CDX-BR16-GFP, NSG-LM2-GFP and NSG-4T1-GFP, n = 7 for BALB/c-4T1-GFP, n = 5 for MMTV-PyMT, error bars, s.e.m. j, Plot showing the number of CTCs in each mouse-model-derived CTC–WBC cluster. The red line represents the mean. k, Pie charts displaying the mean percentage of single CTCs (grey), CTC clusters (green) and CTC–WBC clusters (red) in mice upon blood draw via heart puncture (HP) or tumour-draining vessel (TDV) (left). The plots show the mean number of CTCs from the same experiment (right). l, Plot showing fold change of CTC counts, comparing HP versus TDV blood draw. Error bars, s.e.m.; n = 5 for NSG-CDX-BR16-GFP, n = 3 for NSG-LM2-GFP; P values by two-sided Student’s t-test are shown (k, l). m, Plots showing the number of CTCs in each mouse model-derived CTC–WBC cluster, isolated via HP or TDV. The red lines represent the mean. P values by two-sided Student’s t-test are shown. n, Plot showing the mean number of CTCs at day 10 after tumour inoculation, collected from HP, TDV or peripheral circulation (that is, tail vein; PC). Error bars, s.e.m.; n = 3; P values by two-sided Student’s t-test are shown.
