Extended Data Fig. 8: RORα overexpression enhances experimental OA in mice.
From: The CH25H–CYP7B1–RORα axis of cholesterol metabolism regulates osteoarthritis
a, Representative immunostaining images of RORα and the aggrecan neoepitopes DIPEN and NITEGE in cartilage of mice that received intra-articular injection of Ad-C or Ad-RORα (n = 5 mice per group). b, Representative immunostaining images of RORα in knee joint sections of transgenic and wild-type mice (n = 4 mice each). c, Transgenic and wild-type mice were subjected to sham operation or DMM surgery. Representative safranin-O staining images of joint sections (left) and quantification of OARSI grade, osteophyte maturity and SBP thickness (right; n = 6 mice per group), 6 weeks after sham operation or DMM surgery. Means ± 95% CI with Mann–Whitney U test for OARSI grade and osteophyte maturity; means ± s.e.m. with two-tailed t-test for SBP thickness. Exact P values (for P < 0.001) can be found in the accompanying Source Data. Scale bars, 25 μm.
