Extended Data Fig. 10: Regulation of cholesterol metabolism by OA-associated catabolic signalling in FLSs and Raw264.7 cells.
From: The CH25H–CYP7B1–RORα axis of cholesterol metabolism regulates osteoarthritis
a, Total cholesterol levels in FLSs treated with IL-1β (1 ng ml−1, 36 h) or TNFα (50 ng ml−1, 36 h) or infected with 800 MOI of Ad-C, Ad-HIF-2α or Ad-ZIP8 for 36 h (n = 6). b, mRNA levels in FLSs treated with IL-1β or TNFα or infected with Ad-HIF-2α or Ad-ZIP8 (n = 6). c, mRNA levels of the indicated molecules in FLSs treated with cholesterol or infected with Ad-CH25H, Ad-CYP7B1 or Ad-RORα (n = 6). d, Total cholesterol levels in Raw264.7 cells treated as in a (n = 7). e, mRNA levels of the indicated molecules in Raw264.7 cells treated with IL-1β or TNFα or infected with Ad-C, Ad-HIF-2α or Ad-ZIP8 (n = 7). f, RT–PCR analysis of the indicated molecules in Raw264.7 cells treated with IL-1β or TNFα or infected with Ad-HIF-2α or Ad-ZIP8 (n = 7). g, mRNA levels in Raw264.7 cells treated with cholesterol or infected with Ad-CH25H (n = 6). h, Total cholesterol levels and mRNA levels (n = 6) of the indicated molecules in human normal chondrocytes treated with IL-1β (2.5 ng ml−1, 36 h) or TNFα (50 ng ml−1, 36 h). i, Representative immunostaining images of CH25H, CYP7B1 and RORα in synovial tissue from humans with OA or rheumatoid arthritis (n = 4). Means ± s.e.m. with two-tailed t-test (a, d, h) or with one-way ANOVA and Bonferroni test (b, c, e, g). n indicates the number of biologically independent samples or human specimens. Exact P values (for P < 0.001) can be found in the accompanying Source Data. For gel source data, see Supplementary Fig. 1. Scale bars, 25 μm.
