Extended Data Fig. 1: Purification and cryo-EM of NDH from T. elongatus.
From: Structure of the complex I-like molecule NDH of oxygenic photosynthesis
a, Blue native PAGE gel of NDH solubilized by β-DDM and purified by Ni2+-affinity chromatography with fractions, positions of molecular-weight marker and NDH indicated. b, Size-exclusion chromatography trace of concentrated Ni2+-affinity eluates. The gold bar indicates the fraction that shows a single band corresponding to NDH on the blue native PAGE gel (right). See Supplementary Fig. 1 for source gels for a and b. c, Micrograph of frozen hydrated NDH used in this study, with exemplar particles boxed and 2D-class averages generated from dataset 1 showing clear internal features.
