Extended Data Fig. 2: Cryo-EM data-processing workflow.
From: Structure of the complex I-like molecule NDH of oxygenic photosynthesis
a, b, Schematic of pre-processing, classification and refinement procedures used to generate the maps obtained in this study (see Methods and Supplementary Methods for details) for dataset 1 (a) and dataset 2 (b). Boxed maps indicate those used in model building and solid boxes indicate those used for coordinate refinement. The masks used for focused classification of the X-cofactor are indicated in cyan. The apo- and holo X-cofactor focused maps are enlarged to emphasize the X-site (coloured purple). Resolutions are for an FSC of 0.143, as described in Methods.
