Extended Data Fig. 6: Microglial subtypes in healthy human brains.
From: Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution
a, t-SNE plot of 1,180 human microglia showing the distribution of individual microglia from 5 patients. Each dot represents a single cell. Different colours indicate different patients. b, t-SNE plot of 1,180 individual human microglia isolated from 5 individual non-pathological brains depicts four major clusters (HHu-C1 to HHu-C4). Each dot represents a single cell. Colours correspond to each cluster. c, Heat map showing the distribution of the healthy human clusters in each individual patient. d, Bar graphs representing the relative abundance of microglial cells in the respective clusters from five individual non-pathological brains. Colours represent distinct clusters. e, Heat map of the top differentially regulated genes that were up- or downregulated in each cluster. f, t-SNE plots for CST3, P2RY13, CCL4 and EGR2 mRNA expression. CCL4 and EGR2 are enriched in the cluster HHu-C4.
