Extended Data Fig. 1: Comprehensive analysis of microglial diversity by scRNA-seq.
From: Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution
a, Illustration depicting the workflow for the isolation of microglia from different regions of the CNS of embryonic (E16.5), juvenile (3 weeks of age) and adult (16 weeks of age) mice during homeostasis and during pathology (FNX or cuprizone-mediated demyelination for scRNA-seq). b, t-SNE plot showing 3,826 analysed microglial cells from different conditions tested in this study. Each dot represents a single cell. c, t-SNE plot depicting 13 clusters for all of the different conditions (3,826 cells). Colours represent each cluster. d, t-SNE plots depicting single microglia from the replicates from different regions of the CNS of individual embryos, juvenile and adult mice and diseased mice (3,826 cells).
