Extended Data Fig. 1: VACV-induced 2′,3′-cGAMP degradation is cell-line and tissue-type independent.
From: Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS–STING signalling
a, TLC analysis of the stability of 2′,3′-cGAMP (the 3′–5′ bond is radiolabelled) following incubation in human monocyte (THP-1) or kidney (HEK293T) cytosolic lysates. 2′,3′-cGAMP is highly stable with no degradation detected after >20 h of incubation. b, TLC analysis of VACV-induced 2′,3′-cGAMP degradation. Lysates were prepared from African green monkey (Chlorocebus aethiops (Vero)) and golden hamster (Mesocricetus auratus (BSR-T7)) cells. These lysates exhibit 2′,3′-cGAMP degradation activity after infection with VACV, but not after mock infection (M). c, Time-course analysis of 2′,3′-cGAMP-degradation activity following infection of BSC-40 cells (C. aethiops) with VACV. 2′,3′-cGAMP degradation activity is detectable early <1 h after infection and persists beyond 18 h post-infection. In all panels, the ‘−’ refers to a buffer-only control. All data are representative of three independent experiments.
