Extended Data Fig. 4: Endoderm convergence.
From: A single-cell molecular map of mouse gastrulation and early organogenesis
a, Schematic representing the process of definitive endoderm intercalation following gastrulation, and subsequent gut maturation. Adapted from a previous publication9. b–g, Gene expression levels of Ttr (b), Mixl1 (c), Nkx2-5 (d), Pyy (e), Nepn (f), and Cdx2 (g), overlaid on the Fig. 2a force-directed graph. h, Diffusion map of cells selected for transport map construction; cells selected as termini for pulling mass backward through the transport maps are coloured. i, Results of pushing mass forward through the transport maps are shown on the force-directed layout. j, Violin plots showing expression levels of Trap1a and Rhox5 in all cell-types of the full atlas. k, Dorsal view of a whole-mount fluorescence image of a Ttr::cre; R26R::YFP embryo at E8.5. Green, YFP; red, phalloidin. Arrowhead denotes the increased Ttr–YFP staining in the posterior region of the gut. Scale bar, 300 µm.
