Extended Data Fig. 2: Characterization of reaction products formed during 1O2-mediated oxidation of tryptophan.
From: Singlet molecular oxygen regulates vascular tone and blood pressure in inflammation
a, LC–MS chromatogram of reaction products from the oxidation of l-tryptophan by Rose Bengal and O2 and light, showing molecular ions, [M + H]+ for peaks 1–8. b, MS/MS spectra of precursor ions with m/z ratio of 221 (peaks 2 and 4) and 237 (peaks 5 and 6). c, HPLC chromatograms of reaction products from l-tryptophan oxidation by Rose Bengal and O2 and light, showing retention times of peaks 1–6 monitored at 230 nm and their corresponding post-column chemiluminescence (CL) signals. d, HPLC-UV and chemiluminescence chromatograms of peaks 5 and 6 after their chemical reduction with sodium borohydride. Data shown are representative of six (a) and two independent experiments (b–d).
