Extended Data Fig. 8: Hypocretin and hypocretin receptor-1 expression and production.

a, b, Relative Hcrt mRNA expression in tissues (a; n = 3) and sorted bone marrow cells (b; n = 4). c, Hcrt expression in the bone marrow and bone in Apoe^−/− mice and in Apoe^−/− mice subjected to sleep fragmentation for 16 weeks (n = 5 Apoe^−/− mice; n = 4 Apoe^−/− SF mice). d, Hypocretin-1 protein levels in cerebrospinal fluid (CSF), plasma and bone marrow (BM) fluid of wild-type and Hcrt^−/− mice (n = 4 mice per group). e, Hypocretin-1 protein levels in the plasma and bone marrow fluid of Hcrt^−/− mice 3 h after intra-cisterna magna (i.c.m.) injection of HCRT-1 or PBS. (n = 4 mice per group). f, Relative Hcrtr1 mRNA expression in tissues (n = 4 mice, except aorta and spleen, n = 3). g, Hcrtr2 expression in sorted bone marrow cells (n = 4 mice). h, Granulocyte–macrophage colony forming units (CFU-GM) from bone marrow cells of wild-type mice exposed to hypocretin-1 ex vivo in culture medium (n = 3 per group). i, Assessment of hypocretin receptor-1 protein in hypothalamus and sorted bone marrow neutrophils by western blot. Data are mean ± s.e.m.